Journal: Experimental and Therapeutic Medicine

Article Title: Human adipose-derived mesenchymal stem cells repair cisplatin-induced acute kidney injury through antiapoptotic pathways

doi: 10.3892/etm.2015.2505

Figure Lengend Snippet: Kidney functional recovery and structural repair using human AD-MSCs in animals with acute kidney injury (AKI). (A) Renal function was assessed daily by the levels of BUN, serum Ccr, urinary mALB and β2 mG. Data are presented as mean ± standard error of the mean (SEM). *P<0.01 and **P<0.005, cisplatin + saline group vs. cisplatin + AD-MSCs group. (B) Representative images from H&E and PAS staining of the kidney sections from the healthy control, cisplatin + saline and cisplatin + human AD-MSCs groups (magnification, × 200). (C) Histopathological scoring of cisplatin-induced kidney tubular injury, as indicated with tubular necrosis, cast formation, loss of brush border and intratubular debris. Histopathological scoring was based on the percentage of affected tubules in the kidney sections. Data are presented as the mean ± SEM. **P<0.005, between indicated groups. AD-MSCs, adipose-derived mesenchymal stem cells; BUN, blood urea nitrogen; Ccr, creatinine clearance rate; mALB, microalbumin; β2 mG, microglobulin; U, urinary; H&E, hematoxylin and eosin; PAS, periodic acid-Schiff.

Article Snippet: Rats were housed in metabolism cages (Nanjing Bian Zhen Bio-science Company, Nanjing, China), and the blood serum and urine were collected every 24 h for determination of renal function by biochemistry analysis, which included measurements of the serum blood urea nitrogen (BUN) level (48T/96T; Biofine, Blaine, WA, USA), the creatinine clearance rate (Ccr) (48T/96T; Shanghai Yanjin Bio-science Company, Shanghai, China), urinary microalbumin (mALB) (cat. no. {"type":"entrez-protein","attrs":{"text":"ARB12655","term_id":"1168039453","term_text":"ARB12655"}} ARB12655 ; Beijing Ai Ran Bio-tech Ltd., Beijing, China) and β2 microglobulin (β2 mG) (Shanghai Yuanyan Bio-science Limited Company, Shanghai, China).

Techniques: Functional Assay, Saline, Staining, Control, Derivative Assay

Journal: Toxicological Sciences

Article Title: 3-MCPD 1-Palmitate Induced Tubular Cell Apoptosis In Vivo via JNK/p53 Pathways

doi: 10.1093/toxsci/kfw033

Figure Lengend Snippet: Time course of serum and urine chemistry parameters in Sprague Dawley rats exposed to 1 g/kg of 3-MCPD 1-palmitate. A, Serum urine nitrogen levels; B, serum creatinine levels; C, Kim-1 production in the serum; and D, Kim-1 excretion in the urine were measured at the indicated time points. Different letters represent significant differences at P < .05.

Article Snippet: Serum urea nitrogen and creatinine levels were also measured by Adicon Clinical Laboratories, Inc.

Techniques:

Journal: Toxicological Sciences

Article Title: 3-MCPD 1-Palmitate Induced Tubular Cell Apoptosis In Vivo via JNK/p53 Pathways

doi: 10.1093/toxsci/kfw033

Figure Lengend Snippet: p53 is required for upregulation of proteins, tubular damage, tubular cells apoptosis, and serum chemistry levels induced by 3-MCPD 1-palmitate. A, Western blots analysis of kidney extracts showed increased levels of apoptosis-related proteins bax and cleaved caspase-3 in wild type (WT) C57BL/6 mice but not in the p53−/− mice. Kidney were harvested after 24 h of the treatment and examined with (B, Da) hematoxylin and eosin staining and (C, Db) TUNEL staining as described in the Material and Methods section. The evaluation of tubular damage and tubular cell apoptosis involved averaging the values from 10 fields per kidney under microscope (×200 magnification). (Dc) Serum urea nitrogen and (Dd) creatinine levels were measured in WT and p53−/− mice after 24 h treatment of 3-MCPD 1-palmitate. Different letters represent significant differences at P < .05.

Article Snippet: Serum urea nitrogen and creatinine levels were also measured by Adicon Clinical Laboratories, Inc.

Techniques: Western Blot, Staining, TUNEL Assay, Microscopy

Journal: Toxicological Sciences

Article Title: 3-MCPD 1-Palmitate Induced Tubular Cell Apoptosis In Vivo via JNK/p53 Pathways

doi: 10.1093/toxsci/kfw033

Figure Lengend Snippet: Effects of JNK inhibitor SP600125 on the protein levels, serum chemistry levels and kidney damage induced by 3-MCPD 1-palmitate. SP600125 were administrated to C57BL/6 mice through intraperitoneal injection at doses of 10 and 30 mg/kg body weight 1 h before 3-MCPD 1-palmitate treatment. Twenty-four hours later, kidney was harvested for (A) Western blot analysis. Blood were collected for (Ba) serum urea nitrogen and (Bb) creatinine determination. (Bc, C) Kidney damage were evaluated by using hematoxylin and eosin staining and graded based on the 0–5 Jablonsky grading scale under microscopy (×200 magnification). Different letters represent significant differences at P < .05.

Article Snippet: Serum urea nitrogen and creatinine levels were also measured by Adicon Clinical Laboratories, Inc.

Techniques: Injection, Western Blot, Staining, Microscopy